Morpho-anatomical and biochemical changes associated with rooting of micropropagated ninebark cuttings

Abstract Ninebark ( Physocarpus opulifolius ) is an attractive ornamental shrub with poor rooting characteristics in some cultivars, which a limiting factor commercial production This study was designed to optimize conditions of ninebark cuttings and observe the effect exogenous auxin IBA on morpho-anatomical biochemical changes associated rhizogenesis vitro conditions. Both auxins under study: indole-3-butyric acid (IBA) 1-naphthalene acetic (NAA) gave comparable effects but combination ½ MS + 1 mg·L −1 most cost effective for all parameters. Anatomical at cuttings’ bases during root formation were typical woody plants they accelerated by culture medium. High levels endogenous indole hydrogen peroxide temporarily intensive cell divisions cuttings, polyphenolic contents kept increasing above initial those controls.